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2 edition of vascular organ culture model for genetic and immunological studies of endothelium insitu. found in the catalog.

vascular organ culture model for genetic and immunological studies of endothelium insitu.

Anna Frances Merrick

vascular organ culture model for genetic and immunological studies of endothelium insitu.

by Anna Frances Merrick

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Published by University of Manchester in Manchester .
Written in English


Edition Notes

Thesis (M.D.) - University of Manchester, Faculty of Medicine.

ContributionsUniversity of Manchester. Faculty of Medicine.
The Physical Object
Pagination139p.
Number of Pages139
ID Numbers
Open LibraryOL16435616M

The vascular endothelium, which envelops the circulating blood in a continuous monolayer, is mainly responsible for this the past 20 years numerous other important functions have. The purpose of the present study was to culture human adult endothelial cells (HAECs) on a long-term basis in the laboratory. Previous inability to accomplish this has been the major impediment to the in vitro study of endothelialization of prosthetic grafts with human cells, a problem of significant clinical relevance. We have been successful in developing a technique that allows HAECs from.

Human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMVEC) have been the standards for cell-based assays in the field of angiogenesis research and in antiangiogenic drug discovery. These normal mature endothelial cells may not be most representative of human tumor endothelial cells. Human AC+/CD34+ bone marrow progenitor cells were established in cell. The vascular endothelium represents a dynamic border between circulating blood and the surrounding tissue. This monolayer of endothelial cells (ECs) acts as a nonadhesive surface for platelets and leukocytes and produces a variety of important regulatory factors, such as prostaglandins and NO. 1 In healthy subjects, a low basal level of endothelial turnover, respectively very low amounts of.

The endothelium plays an important role in the regulation of vasomotor tone and the maintenance of vascular integrity. Endothelial dysfunction, i.e., impaired endothelial dependent dilation, is a fundamental component of the pathogenesis of cardiovascular disease. Although endothelial dysfunction is associated with a number of cardiovascular disease risk factors, those risk factors are not the. Uncover the contribution of organ-specific ECs on vascular and organ function; Summary. The endothelium first forms in the blood islands in the extra-embryonic yolk sac and then throughout the embryo to establish circulatory networks that further acquire organ-specific properties during development to support diverse organ functions.


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Vascular organ culture model for genetic and immunological studies of endothelium insitu by Anna Frances Merrick Download PDF EPUB FB2

Organ culture of the rat mesenteric artery branch may therefore be a suitable model for endothelium dysfunction in resistance arteries. Conclusions Organ culture induces a decrease in the NO and prostaglandin-mediated dilatation, while EDHF serves as a backup system to preserve the capability of the artery to respond to a by:   The aim of the present study was to evaluate the suitability of organ culture as a model for endothelium dysfunction.

METHODS: The isometric tension was recorded in isolated segments of the rat mesenteric artery branch, before and after organ culture for 20 h.

Vasodilatation was expressed as % of preconstriction with UCited by: cardiovascular disease. The aim of the present study was to evaluate the suitability of organ culture as a model for endothelium dysfunction. Methods: The isometric tension was recorded in isolated segments of the rat mesenteric artery branch, before and after organ culture for 20 h.

Vasodilatation was expressed as % of preconstriction with UCited by: Endothelium dysfunction is believed to play a role in the development of cardiovascular disease. The aim of the present study was to evaluate the suitability of organ culture as a model for. BACKGROUND: Endothelium dysfunction is believed to play a role in the development of cardiovascular disease.

The aim of the present study was to evaluate the suitability of organ culture as a model for endothelium dysfunction. METHODS: The isometric tension was recorded in isolated segments of the rat mesenteric artery branch, before and after organ culture for 20 h.

Get this from a library. Immunological aspects of the vascular endothelium. [Caroline O S Savage; Jeremy D Pearson;] -- "This is the first volume to look in depth at the functioning of the vascular endothelium, and the diseases and tissue injury that arise as a result of inflammation and immunological responses.

The. human vascular endothelium, a nonfibroblast diploid cell type, may be useful in studies of on-cogenesis and control of the differentiated state. Introduction Infection with simian virus 40 (SV40), an oncogenic member of the papovavirus group, can produce permanent genetic.

Dysfunction of the vascular endothelium is thus a hallmark of human diseases. In this review the main endothelial abnormalities found in various human diseases such as cancer, diabetes mellitus, atherosclerosis, and viral infections are addressed. Key words: Endothelium, Endothelial dysfunction, Atherosclerosis, Stroke, Cancer.

INTRODUCTION. Vascular endothelium — lining the inner side of blood bessels — is one of the largest secretory tissues of the body. Therefore, understanding the cellular and molecular biology of the endothelial cells is essential for the development of new approaches for both the prevention and therapy of cardiovascular diseases.

The vascular endothelium is the inner lining of blood vessels serving as autocrine and paracrine organ that regulates vascular wall function.

Endothelial dysfunction is recognized as initial step in the atherosclerotic process and is well advanced in diabetes, even before the manifestation of end-organ damage.

Strategies capable of assessing changes in vascular endothelium at the preclinical. Most research on vascular endothelium uses human umbilical vein endothelial cells (HUVECs).

First isolated and cultured in the 70s, HUVECs soon became the mainstay of cell-researcher’s work in many laboratories, as they are readily available, quite easy to culture, highly proliferative, and able to migrate and invade new tissues.

Many human studies use species cared for by veterinarians, providing information that may be applied to small animals and that may be used to construct future studies. Conclusion: An organ system itself, the vascular endothelium is an essential component of all organs in the body.

The endothelial cell lining functions to maintain selective. Culture of endothelial cells started two decades ago and is now a useful tool in understanding endothelial physiology and the study of the interaction of endothelial cells with blood cells and various mediators.

In vitro proliferation can be measured by [3H]thymidine incorporation in defined conditions and gives reproducible results. Endothelial cells can be activated by several stimuli. Endothelium dysfunction is believed to play a role in the development of cardiovascular disease. The aim of the present study was to evaluate the suitability of organ culture as a model for endothelium dysfunction.

The isometric tension was recorded in isolated segments of the rat mesenteric artery branch, before and after organ culture for 20 h. The vascular endothelium is the inner lining of blood vessels serving as autocrine and paracrine organ that regulates vascular wall function.

Endothelial dysfunction is recognized as initial step. Organ culture: a new model for vascular endothelium dysfunction By Rikard Alm, Lars Edvinsson and Malin Malmsjö Get PDF (0 MB).

A Genetic Variant Associated with Five Vascular Diseases Is a Distal Regulator of Endothelin-1 Gene Expression Graphical Abstract Highlights d A non-coding variant on chromosome 6 associates with five vascular diseases d Histone acetylation shows a vascular-specific regulatory element at.

Several studies report the key role of the vascular endothelial growth factor (VEGF) signaling on angiogenesis and on tumor growth. This has led to the development of a number of VEGF-targeted agents to treat cancer patients by disrupting the tumor blood vessel supply.

Of them, bevacizumab, an FDA-approved humanized monoclonal antibody against VEGF, is the most promising. culture dishes might exhibit differentiated cell functions, but they commonly fail to mimic tissue-and organ-level structures and functions that are central to disease etiology.

Thus, there is a great need to develop alternative experimental systems containing living human cells that recapitulate tissue- and organ-level pathophysiology in vitro. well suited for the study of activated endothelium e.g. the study of angiogenesis.

Furthermore, as primary cells in culture eventually reach replicative senescence, functional studies of cultured endothelial cells could turn out to be an excellent model system of angiogenesis during ageing.

Vascular progenitor cells and translational research lung alveolar capillary endothelium and renal glomerular fenestrated endothelium had strong expression of CD34, while endothelium from sinusoids of the spleen was negative for CD34, whereas in hepatic sinusoids CD34 expression was restricted to periportal areas [7].Hyperacute rejection is an antibody-mediated cytotoxic response to the fixation of antibodies to specific class I antigens on vascular endothelium, followed by entrapment of formed blood elements.Leukocyte adhesion to endothelium can be measured in static but also in rheologically defined flow conditions.

Normal red blood cells (RBCs) do not adhere to endothelium, while RBC from patients with sickle cell anemia, diabetes mellitus, and malaria have an increased adhesion to endothelium which is mediated by specific VCAM, receptor for.